Dynamic antenna azimuth planning for 3G, 4G and future 5G broadband radio networks

The increasing volume of wireless traffic expected by mobile networks worldwide, means that the wireless industry is searching ways on how to serve the mobile users more efficiently and cost effectively. The mobile users served by any wireless network need to have at least the , required radio coverage to enable the demanded wireless network connectivity while on top the offered network capacity needs to provide the maximum coverage footprint with maximum application throughput. Managing the available capacity in a mobile network is becoming an important technical and financial issue for all mobile operators’ daily operational activities. Any wireless user served, requires high signal to noise and interference ratio (SINR) in order to get optimum service. When SINR is at its best, the radio access network (RAN) offers more revenue and satisfied customers for the mobile operators. In this paper, we focus on how to optimise a mobile network service performance by fine tuning the antenna azimuth headings, such that the geographical coverage area (GEO) of the antenna can serve more users with better radio conditions. Through dynamic antenna azimuth planning, a constant average SINR gain can be achieved. This average SINR gain improves the Key Performance Indicators (KPI) of the network. In principle, a remote azimuth steering system and method is proposed and applied in a real basestation site as a solution to improve the network performance in a self organizing (SON) manner

The Design of Novel Pattern Reconfigurable Antennas for Mobile Networks

This research evaluates a beam reconfigurable basestation transceiver for cellular applications from both a systems and antenna design perspective. The novelty in this research is the investigation of an automatic azimuth beamwidth switching antenna, which can effectively respond to homogeneous traffic distribution in a cellular mobile network. The proposed technique which this antenna uses is azimuth beam switching which incorporates PIN diodes to provide a reconfigurable reflecting ground plane for a three sector antenna. Numerical systems analysis has been carried out on a hexagonal homogeneous cellular network to evaluate how this reconfigurable antenna can balance mean and cell edge capacity through azimuth beamwidth reconfiguration. The optimum azimuth beamwidth is identified as 60°, which achieves the best cell capacity, and by reconfiguring the azimuth beamwidth from 60° to 110°, the maximized capacity at the edges of the cell can be improved. The influence of mechanical tilt, inter site distance, path loss model and vicinity of the cell edge for this antenna are described. This research shows that a mean cell edge improvement from 15Mbit/s to 18Mbit/s is achievable when beamwidth reconfiguration is used, and that this improvement is consistent for cell sizes from 500m to 1500m. Results from a test of an as-manufactured reconfigurable antenna are presented here, and show similar results compared to simulations. To overcome network coverage deterioration at large antenna downtilt angles in a homogeneous cellular mobile network, different beam shaping techniques in the elevation plane, including antenna sidelobe suppressing and null filling, are discussed here. By filling up the first upper-side null for a 12-element antenna array, both the average cell edge and cell capacity can be improved. The application of this beam shaping pattern for a 12-element array is described here, for the purpose of optimising a specific cell within a mobile network which is shown below average coverage and/or capacity. By choosing a proper antenna downtilt angle for this specific cell, whilst keeping the optimum tilt angle for other cells in the network, the cell’s coverage/capacity can be increased without impacting too much on the performance of other surrounding cells. Lastly, the effects of number of antenna elements for a 60° azimuth beamwidth antenna array on the network coverage/capacity are discussed here. This research shows that, as a result of an increasing number of antenna elements in an elevation direction, network capacity can be increased along with the optimum tilt angle. This suggests that a high gain antenna array in a cellular mobile network can be potential for large site deployment and fewer installations

Analysis of causes for poor persistence of CAR-T cell therapy in vivo

Chimeric antigen receptor T-cell (CAR-T-cell) therapy has been well researched to date because of its ability to target malignant tumor cells. The most common CAR-T cells are CD19 CAR-T cells, which play a large role in B-cell leukemia treatment. However, most CAR-T cells are associated with relapse after clinical treatment, so the quality and persistence of CAR-T cells need to be improved. With continuous optimization, there have been four generations of CARs and each generation of CARs has better quality and durability than the previous generation. In addition, it is important to increase the proportion of memory cells in CAR-T cells. Studies have shown that an immunosuppressive tumor microenvironment (TME) can lead to dysfunction of CAR-T cells, resulting in decreased cell proliferation and poor persistence. Thus, overcoming the challenges of immunosuppressive molecules and targeting cytokines in the TME can also improve CAR-T cell persistence. In this paper, we explored how to improve the durability of CAR-T cell therapy by improving the structure of CARs, increasing the proportion of memory CAR-T cells and improving the TME

Demethylating therapy increases cytotoxicity of CD44v6 CAR-T cells against acute myeloid leukemia

BackgroundCD44v6 chimeric antigen receptor T (CD44v6 CAR-T) cells demonstrate strong anti-tumor ability and safety in acute myeloid leukemia (AML). However, the expression of CD44v6 on T cells leads to transient fratricide and exhaustion of CD44v6 CAR-T cells, which affect the application of CD44v6 CAR-T. The exhaustion and function of T cells and CD44v6 expression of AML cells are associated with DNA methylation. Hypomethylating agents (HAMs) decitabine (Dec) and azacitidine (Aza) have been widely used to treat AML. Therefore, there may be synergy between CD44v6 CAR-T cells and HAMs in the treatment of AML.MethodsCD44v6 CAR-T cells pretreated with Dec or Aza were co-cultured with CD44v6+ AML cells. Dec or aza pretreated AML cells were co-cultured with CD44v6 CAR-T cells. The cytotoxicity, exhaustion, differentiation and transduction efficiency of CAR-T cells, and CD44v6 expression and apoptosis in AML cells were detected by flow cytometry. The subcutaneous tumor models were used to evaluate the anti-tumor effect of CD44v6 CAR-T cells combined with Dec in vivo. The effects of Dec or Aza on gene expression profile of CD44v6 CAR-T cells were analyzed by RNA-seq.ResultsOur results revealed that Dec and Aza improved the function of CD44v6 CAR-T cells through increasing the absolute output of CAR+ cells and persistence, promoting activation and memory phenotype of CD44v6 CAR-T cells, and Dec had a more pronounced effect. Dec and Aza promoted the apoptosis of AML cells, particularly with DNA methyltransferase 3A (DNMT3A) mutation. Dec and Aza also enhanced the CD44v6 CAR-T response to AML by upregulating CD44v6 expression of AML cells regardless of FMS-like tyrosine kinase 3 (FLT3) or DNMT3A mutations. The combination of Dec or Aza pretreated CD44v6 CAR-T with pretreated AML cells demonstrated the most potent anti-tumor ability against AML.ConclusionDec or Aza in combination with CD44v6 CAR-T cells is a promising combination therapy for AML patients

Optimization of air distribution mode coupled interior design for civil aircraft cabin

The airflow distribution and thermal comfort of human beings in civil aircraft cabin are influenced by many factors such as the ventilation mode, ventilation air volume, and supply air temperature and so on. Among these factors, the choice of ventilation mode in the civil aircraft cabin is also restricted by the interior and aesthetic designs. Yet few researches noticed the impact of these designs on cabin air distribution. In this paper, an optimization design method for the air distribution mode of civil aircraft will be discussed based on Computational Fluid Dynamic (CFD) method and Micro-Genetic Algorithm (Micro-GA). Two interior design structures with different luggage bin and light band will be used to investigate their influence on the design of air distribution mode. In this optimization, the position of air supply inlets and the supply air angle are defined as the optimization variables. The Predicted Mean Vote (PMV) and the air age are specially chosen as the objective functions. The relevant regulations for cabin temperature uniformity are determined as the thermal constraint conditions. The study results show that the presented method can ensure the convergence of optimization process. The Pareto Optimal Frontiers (POFs) can be obtained from this multi-objective optimization. The POFs can present the relationship of two objective functions. The preferred air distribution mode coupled different interior designs can be efficiently recommended from the optimization results

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Application of the novel-structured multivariable grey model with various orders to forecast the bending strength of concrete

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.Bending strength of concrete is one of the significant indexes to measure the mechanical properties of concrete. A reliable prediction about the bending strength of concrete is of great importance to maintain the health state and service life of concrete. However, it is difficult to obtain reliable data of large samples due to the high cost, serious destructiveness and complex influencing factors of concrete bending strength test data collection. In view of this, based on the multivariable grey prediction model whose modeling object is small data, we construct a new novel-structured multivariable grey prediction model with various orders for predicting the bending strength of concrete. It defines and optimizes the accumulative orders differentially and introduces a nonlinear correction term to expand the model structure. Then, the bending strength of concrete is modeled using the new model, and its comprehensive error is only 0.035 %, which is much smaller than the conventional NSGM(1,N) and FMGM(1,N) models (5.232 % and 2.624 %, respectively). The findings provide a new modeling method for the prediction of concrete bending strength in areas with large temperature difference, and have significance for enriching and improving the methodologies of grey prediction models

Variation of bioactive compounds and 5-hydroxymethyl furfural in coffee beans during the roasting process using kinetics approach

This study investigated the changes in major bioactive compounds and 5-hydroxymethyl furfural (5-HMF) in coffee beans during the roasting process. A Kinetics model was employed to simulate these changes and investigated the thermostability, and the Pearson correlation analysis was applied to indicate the relationship between bioactive compounds and 5-HMF. The research found that three Caffeoylquinic acids (3-CQA, 5-CQA, 4-CQA), and 5-HMF followed a dynamic pattern of increase in the early stage of the roasting process and decrease afterward, which was described by the combination of zero-order and first-order kinetics model. All fitting models fit well and the rate constant (k) of all models follows the Arrhenius law. Therefore, to obtain a higher concentration of bioactive compounds and lower levels of 5-HMF, light or medium roasted coffee beans are recommended. The Pearson correlation analysis indicated that the high levels of 3-CQA, 4-CQA, and caffeine may correspond to higher levels of 5-HMF after roasting

Novel compounds protect auditory hair cells against gentamycin-induced apoptosis by maintaining the expression level of H3K4me2

Aminoglycoside-induced hair cell (HC) loss is a major cause of hearing impairment, and the effective prevention of HC loss remains an unmet medical need. Epigenetic mechanisms have been reported to be involved in protecting cochlear cells against ototoxic drug injury, and in this study we developed new bioactive compounds that have similar chemical structures as the epigenetics-related lysine-specific demethylase 1 (LSD1) inhibitors. LSD1 inhibitors have been reported to protect cochlear cells by preventing demethylation of dimethylated histone H3K4 (H3K4me2). To determine whether these new compounds exert similar protective effects on HCs, we treated mouse cochlear explant cultures with the new compounds together with gentamycin. There was a severe loss of HCs in the organ of Corti after gentamycin exposure, while co-treatment with the new compounds significantly protected against gentamycin-induced HC loss. H3K4me2 levels in the nuclei of HCs decreased after exposure to gentamycin, but H3K4me2 levels were maintained in the presence of the new compounds. Apoptosis is also involved in the injury process, and the new compounds protected the inner ear HCs against apoptosis by reducing caspase-3 activation. Together, our findings demonstrate that our new compounds prevent gentamycin-induced HC loss by preventing the demethylation of H3K4me2 and by inhibiting apoptosis, and these results might provide the theoretical basis for novel drug development for hearing protection